You’ve just finished running an HPLC using a single wavelength in your UV detector, and your HPLC…
You’ve just finished running an HPLC using a single wavelength in your UV detector, and your HPLC trace has two peaks of equal area. Can you say the initial composition was 50:50? What if one component was only 10%, but had an absorption ten times higher than the other component at that analytical wavelength? Apr 24 2022 07:33 AM
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